Scanning fluorescence correlation spectroscopy: a tool for probing microsecond dynamics of surface-bound fluorescent species.

@article{Xiao2005ScanningFC,
  title={Scanning fluorescence correlation spectroscopy: a tool for probing microsecond dynamics of surface-bound fluorescent species.},
  author={Ying Xiao and Volker Buschmann and Kenneth D. Weston},
  journal={Analytical chemistry},
  year={2005},
  volume={77 1},
  pages={36-46}
}
In this report, a combined imaging and fluorescence correlation spectroscopy (FCS) method is described and its ability to characterize microsecond fluctuations in the fluorescence emission of a sample is demonstrated. A sample scanning laser confocal microscope is operated in the customary way while recording the time that each photon is detected with a… CONTINUE READING