Scanning electron microscopy of cells and tissues under fully hydrated conditions.

@article{Thiberge2004ScanningEM,
  title={Scanning electron microscopy of cells and tissues under fully hydrated conditions.},
  author={S. Thiberge and Amotz Nechushtan and David Sprinzak and Opher Gileadi and Vered Behar and Ory Zik and Yehuda Chowers and Shulamit Michaeli and Joseph Schlessinger and Elisha Moses},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2004},
  volume={101 10},
  pages={3346-51}
}
A capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy. The resolution of low-contrast materials is approximately 100 nm, whereas in high-contrast materials the resolution can reach 10 nm. Standard immunogold… CONTINUE READING
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