Scanning electron microscopy of cells and tissues under fully hydrated conditions.

  title={Scanning electron microscopy of cells and tissues under fully hydrated conditions.},
  author={S. Thiberge and Amotz Nechushtan and David Sprinzak and Opher Gileadi and Vered Behar and Ory Zik and Yehuda Chowers and Shulamit Michaeli and Joseph Schlessinger and Elisha Moses},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={101 10},
A capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy. The resolution of low-contrast materials is approximately 100 nm, whereas in high-contrast materials the resolution can reach 10 nm. Standard immunogold… CONTINUE READING
35 Citations
2 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 35 extracted citations


Publications referenced by this paper.
Showing 1-2 of 2 references

A Pp Li Ed Ph Ys Ic a L Sc Ie N Ce S a Pp Li Ed Bi O Lo G Ic a L Sc Ie N Ce S

  • S. Somekh
  • Semiconductor Fabtech,
  • 1999
1 Excerpt

Scanning Electron Microscopy and X-Ray Microanalysis

  • J. I. Goldstein, D. E. Newbury, +5 authors E. Lifshin
  • 1992
2 Excerpts

Similar Papers

Loading similar papers…