Scanning electron microscope imaging of bacteria based on DNA sequence.

@article{Kenzaka2009ScanningEM,
  title={Scanning electron microscope imaging of bacteria based on DNA sequence.},
  author={Takehiko Kenzaka and Ai Ishidoshiro and Katsuji Tani and Masao Nasu},
  journal={Letters in applied microbiology},
  year={2009},
  volume={49 6},
  pages={796-9}
}
AIMS To develop a scanning electron microscopic approach using in situ hybridization (SEM-ISH) for gaining both genetic and morphological information about target bacteria. METHODS AND RESULTS Target cells were hybridized with DNA-targeted polynucleotide probes, and a tyramide signal amplification system was used to increase the sensitivity. The protocol of SEM-ISH enabled to detect low copy number target DNA sequences in individual cells. CONCLUSIONS SEM-ISH allowed the in situ detection… CONTINUE READING
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