Scanning Laser Microscope

  title={Scanning Laser Microscope},
  author={Paul Davidovits and M D Egger},
WE have designed and built a prototype of a scanning microscope for thick objects of low reflectivity and low optical contrast. As light source, we used a 5 mW He–Ne continuous wave laser (Spectra-Physics Model 120). An improved prototype is under construction; in a year or two we expect to have a microscope suitable for advancing studies of brain cells in living animals1–4. 
Scanning laser microscope for biological investigations.
The theory and design of a special purpose scanning laser microscope, particularly suited for biological investigations, is described, intended for the observation of objects embedded within transparent or translucent bodies, such as nerve cells in an intact brain.
Scanning and detection techniques used in a confocal scanning laser microscope
A two‐mirror scanning mechanism for confocal microscopy is described. No optical components, in addition to the scanning mirrors, are used. Design criteria and performance of the scanner are
Scanning Optical Microscopy via a Scanning Electron Microscope
We have described in detail the principles of operation of a commercially available SEM-based scanning optical microscope. The stage consists of a cathodoluminescent material which converts the
Imaging properties and applications of scanning optical microscopes
This review paper is concerned with the imaging properties and major uses of scanning optical microscopes. It is shown that the confocal scanning microscope exhibits a form of super-resolution and
Real-Time Confocal Scanning Microscope — An Optical Instrument with a Better Depth Resolution
In a confocal optical microscope the defocused image disappears instead of blurring, and therefore it is possible to examine different depth sections of a sample. Due to the increased contrast ratio
Fluorescence lifetime measurements using a novel fiber‐optic laser scanning confocal microscope
The novel design of a fiber‐optic laser scanning confocal microscope is described. The optical fiber acts as a flexible light path for both the excitation and return beams and as the confocal pinhole
Confocal optical microscopy
Confocal optical microscopy is a technique for increasing the contrast of microscope images, particularly in thick specimens. By restricting the observed volume, the technique keeps overlying or
Scanning optical microscopes close in on submicron scale
The principles and advantages of the confocal scanning optical microscope (CSOM) are described, and the history of the concept is given. The CSOM is compared with the scanning electron microscope,
Digital scanning laser microscope.
Use of a digital frame memory enabled us to avoid the problem of synchronizing the hardware during data acquisition and data display and makes it possible to ignore any effect of irregular movement of the vibrating stage.
Atomic force fluorescence microscopy : combining the best of two worlds
The complementary strengths and weaknesses of AFM and optical microscopy leads to the desire to integrate both techniques into a single microscope. This thesis describes the development of a


New Reflected-Light Microscope for Viewing Unstained Brain and Ganglion Cells
A new type of reflected-light microscope to form images including only light reflected near the plane of the object based on a mechanical flying-spot technique is designed and constructed.
The tandem scanning reflected light microscope
Reflected-light microscopy of semitransparent material, such as unstained nervous tissue, is usually unsatisfactory because of low contrast and light scattering. In a new microscope both the object
The dip-prism microscope and spike discharges obtained from neuron somata and axons in the cat brain.
The dip-prism microscope has been improved to minimize movement, and monophasic positive spike potentials are always recorded in the region of nerve fibers (axons) in the cat motor cortex.
Fluorescence Changes during Conduction in Nerves Stained with Acridine Orange
These findings suggest that the physicochemical properties of the macromolecules around the dye molecules in the nerve membrane drastically change during the process of nerve conduction.
Progress in Microscopy