Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain

  title={Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain},
  author={Hiroshi Hama and Hiroshi Kurokawa and Hiroyuki Kawano and Ryoko Ando and Tomomi Shimogori and Hisayori Noda and Kiyoko Fukami and Asako Sakaue-Sawano and Atsushi Miyawaki},
  journal={Nature Neuroscience},
Optical methods for viewing neuronal populations and projections in the intact mammalian brain are needed, but light scattering prevents imaging deep into brain structures. We imaged fixed brain tissue using Scale, an aqueous reagent that renders biological samples optically transparent but completely preserves fluorescent signals in the clarified structures. In Scale-treated mouse brain, neurons labeled with genetically encoded fluorescent proteins were visualized at an unprecedented depth in… 
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