Scaffolds for 3D Cell Culture and Cellular Agriculture Applications Derived From Non-animal Sources

@inproceedings{Campuzano2019ScaffoldsF3,
  title={Scaffolds for 3D Cell Culture and Cellular Agriculture Applications Derived From Non-animal Sources},
  author={Santiago Campuzano and Andrew E. Pelling},
  booktitle={Front. Sustain. Food Syst.},
  year={2019}
}
For decades, two-dimensional cell culture has been regarded as a major tool in cellular and molecular biology due to its simplicity, reproducibility and reliable nature. However, it is now recognized that 2D cell culture underrepresents the in vivo environment of living cells. The development and use of 3D scaffolds and biomaterials provide researchers an ability to more closely mimic the in vivo environment. However, many biomaterials are of animal origin, leading to variability, environmental… Expand
22 Citations

Figures from this paper

Homemade Bread: Repurposing an Ancient Technology for in vitro Tissue Engineering
TLDR
It is demonstrated here that mouse fibroblasts, myoblasts and pre-osteoblasts are able to proliferate up to four weeks in culture and control over the mechanical stability of the scaffold can also be achieved with both chemical and enzymatic means. Expand
Bioreactivity of decellularized animal, plant, and fungal scaffolds: perspectives for medical applications.
TLDR
With the integrated evaluation of the scaffold profile in living systems, decellularized animal, plant, and fungal scaffolds have the potential to become essential instruments for safe and controllable biomedical applications. Expand
A Comparative Review of Natural and Synthetic Biopolymer Composite Scaffolds
TLDR
An overview of various natural and synthetic polymers and their possible composite scaffolds with their physicochemical properties including biocompatibility, biodegradability, morphology, mechanical strength, pore size, and porosity are discussed. Expand
Decellularized Plant-Based Scaffolds for Guided Alignment of Myoblast Cells
TLDR
It is concluded that the microtopography of the vascular bundle guides muscle cell alignment in in vitro studies of muscle myogenesis, where structural anisotropy is required to more closely resemble in vivo conditions. Expand
Recent developments in sustainably sourced protein-based biomaterials
TLDR
An overview of the use of natural proteins in biomaterial development and an insight into current research and future directions in each of these areas are given to expand knowledge on the capabilities of sustainably sourced proteins as advanced biomaterials. Expand
Scaffolds for the manufacture of cultured meat.
TLDR
The advantages and disadvantages of scaffold materials and potential scaffolding related to scale-up solution for the production of cultured meat are reviewed and discussed. Expand
Analysis of actin and focal adhesion organisation in U2OS cells on polymer nanostructures
TLDR
The combination of high throughput nanofabrication, advanced optical microscopy, molecular biology tools to visualise cellular processes and data analysis can be used to investigate how cells interact with nanostructured surfaces and will in the future help to create culture substrates that induce particular cell function. Expand
Chitosan Anchored Nanoparticles in Current Drug Development utilizing Computer-Aided Pharmacokinetic Modeling: Case Studies for Target Specific Cancer Treatment and Future Prospective.
TLDR
It is proved that chitosan-drug conjugated nanoparticles (CDNP) can target cancer affected cells with the least attempt to killing the neighbor host cell, which overcomes the dosage form designing problems of complexity in the biological mechanism and cell specificity. Expand
A novel culture method that sustains ERα signaling in human breast cancer tissue microstructures
TLDR
The proposed model system is a new methodology to study ex vivo breast cancer biology, in particular ERα signaling, suitable for interrogating the long-term effects of anti-endocrine drugs in a set-up that closely resembles the original tumor microenvironment. Expand
Muscle stem cell isolation and in vitro culture for meat production: A methodological review.
TLDR
This review discusses comprehensively and in detail each step of cutting-edge methods for cultured meat production and would be meaningful for both academia and industry to prepare for the new era of cellular agriculture. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 157 REFERENCES
Apple Derived Cellulose Scaffolds for 3D Mammalian Cell Culture
TLDR
It is demonstrated that 3D cellulose scaffolds produced by decellularizing apple hypanthium tissue can be employed for in vitro 3D culture of NIH3T3 fibroblasts, mouse C2C12 muscle myoblasts and human HeLa epithelial cells and can achieve cell densities comparable with other natural and synthetic scaffold materials. Expand
Scaffolds for tissue engineering and 3D cell culture.
TLDR
This chapter reviews the principal polymeric materials that are used for the fabrication of scaffolds and the scaffold fabrication processes, with examples of properties and selected applications. Expand
Biofunctionalized Plants as Diverse Biomaterials for Human Cell Culture.
TLDR
It is shown that it is feasible to use plant tissues as an alternative feedstock of scaffolds for mammalian cells and to enable adhesion of human cells on a diverse set of plant tissues with simple biofunctionalization technique. Expand
Customizing the Shape and Microenvironment Biochemistry of Biocompatible Macroscopic Plant-Derived Cellulose Scaffolds.
TLDR
A new preparation and fabrication approach for producing large scale scaffolds with customizable macroscopic structures that support cell attachment and invasion both in vitro and in vivo and provides an important proof that such biomaterials have the potential for numerous applications in tissue engineering. Expand
Biocompatibility of Subcutaneously Implanted Plant-Derived Cellulose Biomaterials
TLDR
The results demonstrate that native cellulose scaffolds are biocompatible and exhibit promising potential as a surgical biomaterial. Expand
Human collagen produced in plants
TLDR
The shortcomings of animal- and cadaver-derived collagens arising from their source diversity and recycled nature are fully overcome in the plant setting, constituting a collagen source ideal for tissue engineering and regenerative medicine applications. Expand
Crossing kingdoms: Using decellularized plants as perfusable tissue engineering scaffolds.
TLDR
The potential of decellularized plants as scaffolds for tissue engineering, which could ultimately provide a cost-efficient, "green" technology for regenerating large volume vascularized tissue mass, is demonstrated. Expand
Chitin Scaffolds in Tissue Engineering
TLDR
The current status of tissue engineering/regenerative medicine research using chitin scaffolds for bone, cartilage and wound healing applications is provided and the key challenges in this field are outlined and the most likely directions for future development are outlined. Expand
Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine
TLDR
This review focuses on the intrinsic properties offered by chitosan and its use in tissue engineering, considering it as a promising alternative for regenerative medicine as a bioactive polymer. Expand
Microenvironment complexity and matrix stiffness regulate breast cancer cell activity in a 3D in vitro model
TLDR
A strict correlation between cell viability and substrate elasticity is observed; in particular, the number of MCF-7 cells decreased constantly with increasing hydrogel elasticity, highlighting the need to adopt more realistic and a priori defined models for in vitro cancer studies. Expand
...
1
2
3
4
5
...