Satisfactory separation and MS-MS spectrometry of six surfactins isolated from Bacillus subtilis natto.

@article{Oka1993SatisfactorySA,
  title={Satisfactory separation and MS-MS spectrometry of six surfactins isolated from Bacillus subtilis natto.},
  author={Kitaro Oka and Tomoya Hirano and Masato Homma and Hikari Ishii and Kazuo Murakami and Shin-ichi Mogami and A Motizuki and Hiroyuki Morita and Koichi Takeya and Hideji Itokawa},
  journal={Chemical & pharmaceutical bulletin},
  year={1993},
  volume={41 5},
  pages={1000-2}
}
We optimized HPLC conditions for the first time to achieve satisfactory separation of six surfactins, two of which were novel, isolated from Bacillus subtilis natto. Peptide sequences were analyzed by sophisticated MS-MS analysis, performed with a pair of two surfactins with different fatty acid substitutions. Fragment peaks were divided into two series; one was a series of the ions which carried fatty acid, and thus were characterized by the fatty acid mass difference, and the other was a… CONTINUE READING

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