Sandwiched zinc-finger nucleases harboring a single-chain FokI dimer as a DNA-cleavage domain.


Zinc-finger nucleases (ZFNs) are a powerful tool for manipulation of genomic DNA. Recently, we reported a new ZFN composed of one artificial zinc-finger protein (AZP) and a single-chain FokI dimer (scFokI) that refines ZFN technology. While AZP-scFokI cleaved DNA specifically around the AZP-target site, several nucleotide positions were cleaved due to the… (More)
DOI: 10.1016/j.bbrc.2009.10.030


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