Sandwich enzyme-linked immunosorbent assay for plasma cholesteryl ester transfer protein concentration.

@article{Chang1999SandwichEI,
  title={Sandwich enzyme-linked immunosorbent assay for plasma cholesteryl ester transfer protein concentration.},
  author={C K Chang and Tim K. Tso and Jean T. Snook and William B Zipf and Rolando A Lozano},
  journal={Clinical biochemistry},
  year={1999},
  volume={32 4},
  pages={257-62}
}
OBJECTIVES Cholesteryl ester transfer protein (CETP) mediates the transfer of HDL cholesterol to apoB-containing lipoproteins. Its mass and activity are increased in several pro-atherogenic conditions. The objective of this study is to develop a cost- and time-effective sandwich ELISA for plasma CETP concentration. DESIGN AND METHODS Monoclonal anti-CETP, TP20, was used as the capture antibody, while the other biotinylated monoclonal anti-CETP, TP2, was used for detection. The results were… CONTINUE READING