Sampling methods for NMR-based metabolomics of Staphylococcus aureus.

  title={Sampling methods for NMR-based metabolomics of Staphylococcus aureus.},
  author={Xiao-He Wu and Hai-Long Yu and Zhao-Yong Ba and Jing-Yu Chen and H Sun and Bei-zhong Han},
  journal={Biotechnology journal},
  volume={5 1},
To select an appropriate sampling method for comparison of metabolite profiles between planktonic and biofilm Staphylococcus aureus using NMR techniques, we evaluated three methods: quenching-centrifugation (QC), filtration-quenching (FQ) and filtration-quenching-lyophilization (FQL). We found differences in metabolite loss, yield, reproducibility and metabolite profile. QC caused severe metabolite leakage and possible decomposition of nucleotides. FQ achieved high yields and reproducibility… CONTINUE READING

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