Sampling and analytical method development for qualitative assessment of airborne mycobacterial species of the Mycobacterium tuberculosis complex.

@article{Schafer1998SamplingAA,
  title={Sampling and analytical method development for qualitative assessment of airborne mycobacterial species of the Mycobacterium tuberculosis complex.},
  author={Millie P Schafer and Joseph E. Fernback and Paul A. Jensen},
  journal={American Industrial Hygiene Association journal},
  year={1998},
  volume={59 8},
  pages={540-6}
}
This article presents a novel, qualitative approach for detecting airborne M. tuberculosis. Culturing or sample purification is not required. A DNA diagnostic method involving the polymerase chain reaction (PCR) coupled to an enzymatically generated color reaction was used for direct detection of M. bovis BCG (Bacillus of Calmette-Guérin), a surrogate for pathogenic M. tuberculosis. Fewer than 10 mycobacteria were detected with no culturing using this bioanalytical method. Analysis was… CONTINUE READING