Sample preparation and gas chromatography of primary fatty acid amides.

@article{Sultana2006SamplePA,
  title={Sample preparation and gas chromatography of primary fatty acid amides.},
  author={Tamanna Sultana and Mitchell Johnson},
  journal={Journal of chromatography. A},
  year={2006},
  volume={1101 1-2},
  pages={278-85}
}
A method for the isolation of bio-active primary fatty acid amides (PFAM's) from total lipid extract by solid-phase extraction (SPE) was developed and validated. The lowest mass of amide to be loaded and recovered by this method was detected as 0.5 microg using 500 mg of normal phase adsorbent. The isolated PFAM's were separated and quantified by GC/MS and percent recoveries were calculated. An HP-5MS column was able to provide base line separation between the saturated and unsaturated PFAM's… CONTINUE READING
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