Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension.

@article{Rabilloud1994SampleAB,
  title={Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension.},
  author={Thierry Rabilloud and C. Audigier Valette and Johnny J. Lawrence},
  journal={Electrophoresis},
  year={1994},
  volume={15 12},
  pages={1552-8}
}
We describe a modification in the sample application mode for isoelectric focusing with immobilized pH gradients. Instead of being applied at the surface of the gel in a sample cup, the sample is introduced into the gel during the immobilized pH gradient strip rehydration step. This modification implies the use of low percentage gels (below 3.5% T) and specially designed, but simple, rehydration chambers. The main advantages are a uniform resolution without side effects and the possibility of… CONTINUE READING