Salt-dependent studies of NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii

@article{Madern2004SaltdependentSO,
  title={Salt-dependent studies of NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii},
  author={Dominique Madern and M{\'o}nica Camacho and Adoraci{\'o}n Rodr{\'i}guez-Arnedo and Mar{\'i}a-Jos{\'e} Bonete and Giuseppe Zaccai},
  journal={Extremophiles},
  year={2004},
  volume={8},
  pages={377-384}
}
The salt-dependent stability of recombinant dimeric isocitrate dehydrogenase [ICDH; isocitrate: NADP oxidoreductase (decarboxylating), EC 1.1.1.42] from the halophilic archaeon Haloferax volcanii (Hv) was investigated in various conditions. Hv ICDH dissociation/deactivation was measured to probe the respective effect of anions and cations on stability. Surprisingly, enzyme stability was found to be mainly sensitive to cations and very little (or not) sensitive to anions. Divalent cations… CONTINUE READING
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