Sal k 5, a member of the widespread Ole e 1-like protein family, is a new allergen of Russian thistle (Salsola kali) pollen.

@article{Castro2014SalK5,
  title={Sal k 5, a member of the widespread Ole e 1-like protein family, is a new allergen of Russian thistle (Salsola kali) pollen.},
  author={Laura V Castro and Salvador Mas and Rodrigo Barderas and Carlos Col{\'a}s and Javier Garc{\'i}a-Sell{\'e}s and Domingo Barber and R. Walter Rodr{\'i}guez and Mayte Villalba},
  journal={International archives of allergy and immunology},
  year={2014},
  volume={163 2},
  pages={142-53}
}
BACKGROUND Salsola kali is an Amaranthaceae weed with important repercussions for pollinosis in temperate areas. Ole e 1-like members are relevant allergens in pollen from different species. We aimed to characterize and produce as recombinant allergen S. kali Ole e 1-like protein. METHODS The natural allergen was purified at homogeneity after three chromatographic steps. Specific cDNA was sequenced and expressed in Pichia pastoris yeast. Structural relationships of natural and recombinant… CONTINUE READING

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