Safranine fluorescent staining of wood cell walls.

  title={Safranine fluorescent staining of wood cell walls.},
  author={Joseph Bond and Lloyd Donaldson and Shante Hill and Kristin Hitchcock},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  volume={83 3-4},
Safranine is an azo dye commonly used for plant microscopy, especially as a stain for lignified tissues such as xylem. Safranine fluorescently labels the wood cell wall, producing green/yellow fluorescence in the secondary cell wall and red/orange fluorescence in the middle lamella (ML) region. We examined the fluorescence behavior of safranine under blue light excitation using a variety of wood- and fiber-based samples of known composition to interpret the observed color differentiation of… CONTINUE READING
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