Safety assessment of the calcium-binding protein, apoaequorin, expressed by Escherichia coli.

  title={Safety assessment of the calcium-binding protein, apoaequorin, expressed by Escherichia coli.},
  author={Daniel L Moran and Afua O Tetteh and Richard E. Goodman and Mark Y. Underwood},
  journal={Regulatory toxicology and pharmacology : RTP},
  volume={69 2},
Subchronic toxicity of lyophilized apoaequorin protein powder in Sprague-Dawley rats
The no-observed-adverse-effect-level (NOAEL) of lyophilized apoaequorin protein powder (LAPP) was 4000 mg/kg/day, the highest dose tested in male and female rats, considered to be of little toxicological significance.
Bioactive Compounds of Nutraceutical Value from Fishery and Aquaculture Discards
The possible valorisation of seafood and their by-products are focused on, which represent a source of biomolecules, useful for the sustainable production of high-value nutraceutical compounds in the authors' circular economy era.
Nutraceuticals for Cognitive Dysfunction
Various nutraceuticals and substances that have potential to improve cognition and memory in senior dogs and cats are described.
Bad Science: Exploring the unethical research behind a putative memory supplement
Students who successfully complete the lesson know criteria for evaluating the quality of material that is presented as scientific and feel empowered to make informed decisions about health and wellness based on their newly acquired practice with identifying valid/invalid scientific reasoning and with recognizing pseudoscience.
Food safety assessment of Cry8Ka5 mutant protein using Cry1Ac as a control Bt protein.
  • D. FariasM. P. Viana A. F. Carvalho
  • Biology, Medicine
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2015


Calcium-binding proteins: basic concepts and clinical implications.
  • C. Heizmann
  • Biology
    General physiology and biophysics
  • 1992
Another protein family are the annexins, members of which interact with phospholipids and cellular membranes in a calcium-dependent manner, and in some cases members of the annexin family were even found to interact with EF-hand proteins.
A multi-laboratory evaluation of a common in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins.
Calcium-binding proteins.
Safety assessment of Apoaequorin, a protein preparation: subchronic toxicity study in rats.
Measurement of cytosolic free calcium in mammalian cells with aequorin.
Evidence is presented that the aequorin-Ca light signal originates in the cytosol and that the cells subjected to the HOST procedure have a normal viability and functional integrity with respect to growth, respiration, membrane transport calcium metabolism, and hormone responsiveness.
Stability of food allergens to digestion in vitro
The data are consistent with the hypothesis that food allergens must exhibit sufficient gastric stability to reach the intestinal mucosa where absorption and sensitization can occur, and indicate the stability to digestion is a significant and valid parameter that distinguishes food allergen from nonallergens.
Crystal Structure of a Ca2+-discharged Photoprotein
The crystal structure of the Ca2+-discharged photoprotein obelin is reported and a significant repositioning and flipping of the His-175 imidazole ring as crucially required in the trigger hypothesis are lent support to the proposed mechanisms and provide new structural insight into details of these processes.
All three Ca2+‐binding loops of photoproteins bind calcium ions: The crystal structures of calcium‐loaded apo‐aequorin and apo‐obelin
There are easily discerned shifts in both helix and loop regions, and it is suggested that these subtle shifts are the basis of the ability of these photoproteins to sense Ca2+ concentration transients and to produce their bioluminescence response on the millisecond timescale.
Purification of the Major Allergen of Red Soft Coral (Dendronephthya nipponica)
It is found that RSC has a strong sensitizing activity in humans at a Molecular weight of 10 kD or more, while it has no IgE-producing activity at a molecular weight of less than less than 10KD.