Safe separation of sputum cells from mucoid glycoprotein.

@article{Tockman1995SafeSO,
  title={Safe separation of sputum cells from mucoid glycoprotein.},
  author={Melvyn S. Tockman and You-lin Qiao and Ling Li and G Z Zhao and Ramaswamy Sharma and L L Cavenaugh and Yener S. Erozan},
  journal={Acta cytologica},
  year={1995},
  volume={39 6},
  pages={
          1128-36
        }
}
OBJECTIVE To compare the safety and efficacy of homogenizing sputum with deoxyribonuclease I (DNAse I), dithiothreitol (DTT), N-acetyl-L-cysteine, sodium EDTA and trypsin against standard mechanical blending to provide mucus-free, single-cell suspensions for quantitative analysis. STUDY DESIGN Clinical sputum specimens or cultured human bronchogenic carcinoma cells were preserved in 2% polyethylene glycol/50% ethanol, divided into aliquots, counted and stained (Papanicolaou and avidin-biotin… CONTINUE READING

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A case-cohort study of an early biomarker of lung cancer in a screening cohort of Yunnan tin miners in China.

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