Saccharomyces cerevisiae recA homologues RAD51 and DMC1 have both distinct and overlapping roles in meiotic recombination

@article{Shinohara1997SaccharomycesCR,
  title={Saccharomyces cerevisiae recA homologues RAD51 and DMC1 have both distinct and overlapping roles in meiotic recombination},
  author={Akira Shinohara and Stephen L. Gasior and Tomoko Ogawa and Nancy Kleckner and Douglas K. Bishop},
  journal={Genes to Cells},
  year={1997},
  volume={2}
}
Rad51 and Dmc1 are Saccharomyces cerevisiae homologues of the Escherichia coli recombination protein RecA. Mutant analysis has shown that both proteins are required for normal meiotic recombination, for timely and efficient formation of synaptonemal complex and for normal progression out from meiotic prophase. 
Roles of RecA homologues Rad51 and Dmc1 during meiotic recombination
TLDR
In this review, recent advances on the knowledge about the roles of Rad51 and Dmc1 during meiosis are summarized and discussed. Expand
Functions of a New Protein Complex Involved in Homologous Recombination during Meiosis
TLDR
The function of Dmc1-containing protein machinery in meiotic-recombination and possible asymmetric distribution of the two RecA homologues on chromosomes are discussed. Expand
High copy number suppression of the meiotic arrest caused by a dmc1 mutation: REC114 imposes an early recombination block and RAD54 promotes a DMC1‐independent DSB repair pathway
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Two genes are isolated which, when present at high copy numbers, suppress the meiotic arrest phenotype conferred by dmc1 mutations. Expand
Crossover interference in Saccharomyces cerevisiae requires a TID1/RDH54- and DMC1-dependent pathway.
TLDR
Tetrad analysis is used to show that meiotic recombination in RAD54-suppressed dmc1Delta cells is similar to that in tid1; the frequency of COs and gene conversions is near normal, but crossover interference is defective, which support the proposal that crossover interference acts at the strand invasion stage of recombination. Expand
Rad51 Is an Accessory Factor for Dmc1-Mediated Joint Molecule Formation During Meiosis
TLDR
Meiotic recombination in budding yeast requires two RecA-related proteins, Rad51 and Dmc1, both of which form filaments on DNA capable of directing homology search and catalyzing formation of homologous joint molecules (JMs) and strand exchange, and Rad51 is a multifunctional protein that catalyzes recombination directly in mitosis and indirectly, via Dmc2 during meiosis. Expand
Recombination at work for meiosis.
In sexually reproducing organisms, homologous recombination increases genetic diversity in gametes and ensures proper chromosome segregation. Recent publications have provided details of theExpand
Rad61/Wpl1 (Wapl), a cohesin regulator, controls chromosome compaction during meiosis
TLDR
It is shown that meiotic chromosome axes are shortened in the budding yeast rad61/wpl1 mutant, suggesting that Rad61/Wpl1 negatively regulates chromosome axis compaction, which is required for efficient resolution of telomere clustering during meiosis I, indicating a positive effect of Rad61 or Wpl1 on the cohesin function required for telomeres dynamics. Expand
Inhibition of the Smc5/6 Complex during Meiosis Perturbs Joint Molecule Formation and Resolution without Significantly Changing Crossover or Non-crossover Levels
TLDR
Results suggest that the Smc5/6 complex aids primarily in the resolution of joint molecules formed outside of canonical inter-homolog pathways, as well as return-to-function studies indicate that it performs its most important functions during joint molecule resolution without influencing crossover formation. Expand
Genetic and cytological characterization of the RecA-homologous proteins Rad51 and Dmc1 of Schizosaccharomyces pombe
TLDR
The functional defects of terminally epitope-tagged Dmc1 and Rad51 are described and discussed in terms of protein interaction and it is demonstrated that the reduction in meiotic recombination in dmc1 mutants is not caused by a disturbance of rad24 expression from the dMC1-rad24 bicistronic RNA. Expand
VDE‐initiated intein homing in Saccharomyces cerevisiae proceeds in a meiotic recombination‐like manner
TLDR
This work has shown that during meiosis of the yeast Saccharomyces cerevisiae, the site‐specific endonuclease encoded by VMA1 intein, VDE, triggers a single double‐strand break at an inteInless allele, leading to VMA 1 inte in homing. Expand
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