Saccharomyces cerevisiae acquires resistance to 2-deoxyglucose at a very high frequency.

  title={Saccharomyces cerevisiae acquires resistance to 2-deoxyglucose at a very high frequency.},
  author={M F Heredia and Claudio F. Heredia},
  journal={Journal of bacteriology},
  volume={170 6},
We have found that Saccharomyces cerevisiae acquires spontaneously increasing resistance to 2-deoxyglucose at a very high frequency. This finding allows the easy isolation of different types of resistant strains of interest for metabolic studies with 2-deoxyhexoses. On the other hand, it sounds a note of caution in the widespread use of 2-deoxyglucose as a selective agent for the isolation of yeast mutants with impaired hexose transport or phosphorylation systems. 

From This Paper

Figures, tables, and topics from this paper.

Explore Further: Topics Discussed in This Paper


Publications citing this paper.
Showing 1-6 of 6 citations


Publications referenced by this paper.
Showing 1-10 of 14 references

Catabolite repression mutants of yeast

  • C. F. Heredia
  • FEMS Microbiol . Rev .
  • 1986

Mechanism of mannose toxicity

  • C. Gancedo
  • Biochem . Biophys . Res . Commun .
  • 1986

Sugar transport and hexose - ATP kinase activity in a 2 - deoxyglucose tolerant mutant of yeast Rhodotorula glutinis

  • C. F. Heredia
  • J . Gen . Microbiol .
  • 1985

Use of glucose analogues to study the mechanism of glucose-mediated cAMP increase in yeast

  • P. Eraso, J. M. Gancedo
  • 1985
2 Excerpts

Characterization of a phosphatase specific for 2 - deoxyglucose - 6 - phosphate in a yeast mutant

  • T. Platt
  • FEBS Lett .
  • 1977

Assay of inorganic phosphate, total phosphate and phosphatases

  • B. N. Ames
  • Methods Enzymol
  • 1966
1 Excerpt

Resistance to 2 - deoxyglucose in a yeast mutant

  • N. J. Rosebrough, A. L. Farr, R. J. Randall
  • Biochim . Biophys . Acta
  • 1964