Saccharomyces cerevisiae Bat1 and Bat2 Aminotransferases Have Functionally Diverged from the Ancestral-Like Kluyveromyces lactis Orthologous Enzyme

@article{Coln2011SaccharomycesCB,
  title={Saccharomyces cerevisiae Bat1 and Bat2 Aminotransferases Have Functionally Diverged from the Ancestral-Like Kluyveromyces lactis Orthologous Enzyme},
  author={Maritrini Col{\'o}n and F Hern{\'a}ndez and Karla L{\'o}pez and H{\'e}ctor Quezada and James Gonz{\'a}lez and Geovani Dom{\'i}nguez L{\'o}pez and Cristina Aranda and Alicia Gonz{\'a}lez},
  journal={PLoS ONE},
  year={2011},
  volume={6}
}
Background Gene duplication is a key evolutionary mechanism providing material for the generation of genes with new or modified functions. The fate of duplicated gene copies has been amply discussed and several models have been put forward to account for duplicate conservation. The specialization model considers that duplication of a bifunctional ancestral gene could result in the preservation of both copies through subfunctionalization, resulting in the distribution of the two ancestral… Expand
Evolutionary Diversification of Alanine Transaminases in Yeast: Catabolic Specialization and Biosynthetic Redundancy
TLDR
The study shows that the ancestral alanine transaminase function has been retained by the ScALT1 encoded enzyme, which has specialized its catabolic character, while losing theAlanine independent role observed in the ancestral type enzymes. Expand
In Kluyveromyces lactis a Pair of Paralogous Isozymes Catalyze the First Committed Step of Leucine Biosynthesis in Either the Mitochondria or the Cytosol
TLDR
Analysis of selected duplicated pairs has elucidated some of the mechanisms underlying the functional diversification of Saccharomyces cerevisiae paralogous genes, and KlLEU4 could be considered as the functional ortholog of ScLEu4, since its encoded isozyme can complement both the Scleu4Δ ScleU9Δ leucine auxotrophy and the ScLeu4 δ ScLEU9 complex phenotype. Expand
Diversification of Transcriptional Regulation Determines Subfunctionalization of Paralogous Branched Chain Aminotransferases in the Yeast Saccharomyces cerevisiae
TLDR
The presented results confirm that BAT1 expression is determined by transcriptional activation through the action of the Leu3–α-isopropylmalate active isoform, and uncovers the existence of a novel α-IPM biosynthetic pathway operating in a put3Δ mutant grown on VIL, through Bat2-Leu2- Leu1 consecutive action. Expand
Functional roles of a predicted branched chain aminotransferase encoded by the LkBAT1 gene of the yeast Lachancea kluyveri.
TLDR
The proposition that the BCAT function in ancestral-type yeasts has been distributed in the two paralogous genes present in S. cerevisiae is supported. Expand
Paralogous ALT1 and ALT2 Retention and Diversification Have Generated Catalytically Active and Inactive Aminotransferases in Saccharomyces cerevisiae
TLDR
Results indicate that alanine could act as ALT2 Nrg1-co-repressor, indicating a role inAlanine biosynthesis, although the encoded-protein has no alAnine aminotransferase enzymatic activity. Expand
Saccharomyces cerevisiae Differential Functionalization of Presumed ScALT1 and ScALT2 Alanine Transaminases Has Been Driven by Diversification of Pyridoxal Phosphate Interactions
TLDR
Structural organization and pyridoxal phosphate (PLP) binding properties of ScAlt1 and ScAlt2 are analyzed indicating functional diversification could have determined loss of Sc Alt2 alanine transaminase activity and thus its role in alanines metabolism. Expand
Duplication and Functional Divergence of Branched-Chain Amino Acid Biosynthesis Genes in Aspergillus nidulans
TLDR
While both β-isopropylmalate dehydrogenase genes act in leucine biosynthesis, the two most highly expressed BCAA aminotransferases are responsible for BCAA biosynthesis. Expand
A blueprint of the amino acid biosynthesis network of hemiascomycetes.
TLDR
The results argue for careful use of information from S. cerevisiae and for joint efforts to fill the knowledge gaps in other species, and suggest that αIPMS and BCAA aminotransferase are solely located in the cytosol of P. pastoris, requiring correction of the leucine biosynthesis pathway layout in this species. Expand
The Zygosaccharomyces bailii transcription factor Haa1 is required for acetic acid and copper stress responses suggesting subfunctionalization of the ancestral bifunctional protein Haa1/Cup2
TLDR
The transcription factor ZbHaa1 is required for adaptive response and tolerance to both acetic acid and copper stresses and the subfunctionalization of the single ancestral Haa1/Cup2 orthologue that originated HAA1 and Cup2 paralogues after whole genome duplication is proposed. Expand
Valine biosynthesis in Saccharomyces cerevisiae is regulated by the mitochondrial branched-chain amino acid aminotransferase Bat1
TLDR
It is found that intracellular valine content was dramatically decreased in Δbat1 cells, whereas Δbat2 cells exhibited no changes in the valine level, which suggests that the mitochondria are the major site of valine biosynthesis, and mitochondrial BCAT is important for valinesynthesis in S. cerevisiae. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 40 REFERENCES
Gene duplication and the adaptive evolution of a classic genetic switch
TLDR
It is suggested that duplication of the ancestral bifunctional gene allowed for the resolution of an adaptive conflict between the transcriptional regulation of the two gene functions and became one of the most tightly regulated genes in the genome. Expand
Molecular evidence for an ancient duplication of the entire yeast genome
TLDR
A model is proposed in which this species is a degenerate tetraploid resulting from a whole-genome duplication that occurred after the divergence of Saccharomyces from Kluyveromyces, and protein pairs derived from this duplication event make up 13% of all yeast proteins. Expand
Swi/SNF‐GCN5‐dependent chromatin remodelling determines induced expression of GDH3, one of the paralogous genes responsible for ammonium assimilation and glutamate biosynthesis in Saccharomyces cerevisiae
TLDR
The results show that GDH3 expression is repressed in glucose‐grown cultures, as opposed to what has been observed for GDH1, and induced under respiratory conditions, or under stationary phase, highlighting the fact that operation of facultative metabolism requires strict control of enzymes, like Gdh3p, involved in biosynthetic pathways that use tricarboxylic acid cycle intermediates. Expand
Proof and evolutionary analysis of ancient genome duplication in the yeast Saccharomyces cerevisiae
TLDR
It is shown that the yeast Saccharomyces cerevisiae arose from ancient whole-genome duplication, by sequencing and analysing Kluyveromyces waltii, a related yeast species that diverged before the duplication. Expand
Mitochondrial and Cytosolic Branched-chain Amino Acid Transaminases from Yeast, Homologs of the myc Oncogene-regulated Eca39 Protein*
TLDR
The severe growth defect of the double deletion mutant observed even in the presence of branched-chain amino acids suggests that the Bat proteins, in addition to the supply of these amino acids, perform another important function in the cell. Expand
The evolution of functionally novel proteins after gene duplication
  • A. Hughes
  • Biology, Medicine
  • Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 1994
TLDR
A model for the development of new protein is proposed under which a period of gene sharing ordinarily precedes the evolution of functionally distinct proteins. Expand
Specialization of the paralogue LYS21 determines lysine biosynthesis under respiratory metabolism in Saccharomyces cerevisiae.
TLDR
It can be concluded that retention and further biochemical specialization of the LYS20- and LYS21-encoded enzymes with partially overlapping roles contributed to the acquisition of facultative metabolism. Expand
Saccharomyces cerevisiae has a single glutamate synthase gene coding for a plant-like high-molecular-weight polypeptide
TLDR
The results show that S. cerevisiae has a single NADH-GOGAT enzyme, consisting of three 199-kDa monomers, that differs from the one found in prokaryotic microorganisms but is similar to those found in other eukaryotic organisms such as alfalfa. Expand
Preservation of duplicate genes by complementary, degenerative mutations.
TLDR
Focusing on the regulatory complexity of eukaryotic genes, it is shown how complementary degenerative mutations in different regulatory elements of duplicated genes can facilitate the preservation of both duplicates, thereby increasing long-term opportunities for the evolution of new gene functions. Expand
Regulation of primary carbon metabolism in Kluyveromyces lactis.
TLDR
First insight into the molecular basis for the different regulatory strategies is beginning to emerge from comparative studies on S. cerevisiae and K. lactis, suggesting that different physiological responses result from modified interactions of similar molecular modules. Expand
...
1
2
3
4
...