SYNAPTOSOMAL PHOSPHORYLATION OF D‐GLUCOSAMINE

@article{Tan1978SYNAPTOSOMALPO,
  title={SYNAPTOSOMAL PHOSPHORYLATION OF D‐GLUCOSAMINE},
  author={C. H. Tan and E. Raghupathy and Neal A. Peterson},
  journal={Journal of Neurochemistry},
  year={1978},
  volume={31}
}
Abstract— Synaptosomal fractions from rat brains can phosphorylate d‐glucosamine once it is transported across the synaptosomal membrane. Intrasynaptosomal phosphorylation of d‐glucosamine was rapid; up to 80% of the d‐glucosamine was recovered as the phosphorylated amino sugar during 15 min of incubation with the substrate. Transport of d‐glucosamine was the rate‐limiting step in the uptake process since in the presence of cytochalasin B, a specific sugar transport inhibitor, the uptake of d… Expand
1 Citations
The uptake of 3‐deoxy‐3‐fluoro‐D‐Glucose by synaptosomes from rat brain cortex
TLDR
The recorded 3‐deoxy‐3‐fluoro‐D‐glucose influx is slightly reduced by potassium cyanide, antimycin A, 2,4‐dinitrophenol, and rotenone, and the uptake reduction caused by these four reagents is relieved by the addition of exogenous ATP. Expand

References

SHOWING 1-10 OF 16 REFERENCES
CHARACTERISTICS OF D‐GLUCOSAMINE UPTAKE BY RAT BRAIN SYNAPTOSOMES
TLDR
The rate of D‐glucosamine uptake, after correcting for simple diffusion, obeys Michaelis‐Menten kinetics and is sensitive to inhibition by several sulfhydryl reagents, thus implying the involvement of sulfHydryl groups in the transport process. Expand
HIGH‐AFFINITY TRANSPORT AND PHOSPHORYLATION OF 2‐DEOXY‐d‐GLUCOSE IN SYNAPTOSOMES 1,2
TLDR
2‐Deoxy‐d‐glucose (2 DG) entered synaptosomes (from rat brain) by a high‐affinity, Na+‐independent glucose transport system and probably is a special property of neuronal cell membranes and could be useful in helping to distinguish separated neurons from glial cells. Expand
INCORPORATION OF [14C]GLUCOSAMINE INTO SYNAPTOSOMES IN VITRO 1
TLDR
Radioactivity was demonstrable in the proteins as sialic acid by paper chromatography and specific enzymic digestion; and as glucuronic acid by chromatography, electrophoresis, and digestion with hyaluronidase. Expand
EFFECTS OF CYTOCHALASIN B ON THE UPTAKES OF MONOSACCHARIDES BY RAT BRAIN SYNAPTOSOMES
TLDR
The resultant non‐saturable cytochalasin‐insensitive uptake rates obtained were found to be widely different among the sugars studied, and must be corrected for in order to estimate accurate kinetic constants of the saturable processes. Expand
Nonspecific incorporation of glucosamine into rat brain synaptosomes and endoplasmic reticulum
Rat brain synaptosomes and endoplasmic reticulum apparently incorporate radioactive glucosamine, in vitro, into trichloroacetic acid-insoluble, non-lipid material, in a time-dependent manner. ThisExpand
D‐GALACTOSE TRANSPORT BY SYNAPTOSOMES ISOLATED FROM RAT BRAIN
TLDR
Galactose was rapidly converted in synaptosomes to phos‐phorylatcd intermediates and was more slowly oxidized to 14CO2 as well as being inhibited by high concentrations of glucose. Expand
D-Glucosamine uptake by rat brain synaptosomes.
TLDR
Uptake of d -glucosamine by rat brain synaptosomes occurs via a saturable transport process which was clearly distinguishable from simple diffusion. Expand
Acetoxycycloheximide effect on synthesis and metabolism of glucosamine-containing macromolecules in brain and in nerve endings.
TLDR
Evidence suggesting reversible incorporation and removal of some carbohydrate residues of carbohydrate-containing macromolecules of brain was found and the possible regulatory role of this process is considered. Expand
(14C)Glucosamine incorporation into specific products in the nerve ending fraction in vivo and in vitro.
TLDR
The results indicate that some [14C]glucosamine is incorporated into specific products by components of the nerve ending fraction and that this glycosylation cannot readily be attributed to contamination with other particles containing Glycosyl transferases. Expand
INCORPORATION OF RADIOACTIVE GLUCOSAMINE INTO MACROMOLECULES AT NERVE ENDINGS 1
  • S. Barondes
  • Chemistry, Medicine
  • Journal of neurochemistry
  • 1968
TLDR
The data suggests that, unlike protein which is largely transported to nerve endings in the axoplasm, there is extensive incorporation of carbohydrate into macromolecules in nerve endings. Expand
...
1
2
...