SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability.

Abstract

Real-time quantitative PCR is used routinely for the high-throughput diagnosis of viral pathogens, such as West Nile virus (WNV). Rapidly evolving RNA viruses present a challenge for diagnosis because they accumulate mutations that may render them undetectable. To explore the effect of sequence variations on assay performance, we generated every possible… (More)

Topics

6 Figures and Tables

Cite this paper

@article{Papin2004SYBRGR, title={SYBR green-based real-time quantitative PCR assay for detection of West Nile Virus circumvents false-negative results due to strain variability.}, author={James F. Papin and Wolfgang Vahrson and Dirk P. Dittmer}, journal={Journal of clinical microbiology}, year={2004}, volume={42 4}, pages={1511-8} }