SYBR Green real-time telomeric repeat amplification protocol for the rapid quantification of telomerase activity.

@article{Wege2003SYBRGR,
  title={SYBR Green real-time telomeric repeat amplification protocol for the rapid quantification of telomerase activity.},
  author={Henning Wege and Michael S Chui and Hai Thanh Le and J. Hear Mass Tran and Mark A. Zern},
  journal={Nucleic acids research},
  year={2003},
  volume={31 2},
  pages={E3-3}
}
The sensitive telomeric repeat amplification protocol (TRAP) permits telomerase detection in mammalian cell and tissue extracts with very low telomerase activity levels. Unfortunately, conventional TRAP assays require complex post-amplification procedures, such as polyacrylamide gel electrophoresis and densitometry, to measure telomerase products. Therefore, a real-time quantitative TRAP assay (RQ-TRAP) was optimized in the present study and evaluated in comparison with a commercially available… CONTINUE READING