SWTY--a general peptide probe for homogeneous solution binding assay of 14-3-3 proteins.

@article{Wu2006SWTYaGP,
  title={SWTY--a general peptide probe for homogeneous solution binding assay of 14-3-3 proteins.},
  author={Meng Wu and Brian Coblitz and Sojin Shikano and Shunyou Long and Lisa M Cockrell and Haian Fu and Min Li},
  journal={Analytical biochemistry},
  year={2006},
  volume={349 2},
  pages={186-96}
}
Dimeric 14-3-3 proteins exert diverse functions in eukaryotes by binding to specific phosphorylated sites on diverse target proteins. Critical to the physiological function of 14-3-3 proteins is the wide range of binding affinity to different ligands. The existing information of binding affinity is mainly derived from nonhomogeneous-based methods such as surface plasmon resonance and quantitative affinity precipitation. We have developed a fluorescence anisotropy peptide probe using a… CONTINUE READING