STUDIES ON THE ENZYME ENOLASE*

@inproceedings{Wold2003STUDIESOT,
  title={STUDIES ON THE ENZYME ENOLASE*},
  author={F. Wold and C. Ballou},
  year={2003}
}
Enolase catalyzes the interconversion of u-glyceric acid 2-phosphat,e and enolpyruvic acid phosphate,’ and, as a participant of Embden-Meyerhof glycolysis and fermentation, is widely distributed in living cells. The enzyme was first purified by Warburg and Christian (1)) who demonstrated its metal activation and made the initial studies of the kinetics and the thermodynamics of the enolase reaction. More recently, Malmstriim (2-6) has extended that work, with special emphasis on the effect of… Expand

Figures and Tables from this paper

Biochemical and Structural Characterization of Enolase from Chloroflexus aurantiacus: Evidence for a Thermophilic Origin
TLDR
The purification and biochemical and structural characterization of enolase from Chloroflexus aurantiacus, a thermophilic anoxygenic phototroph affiliated with the green non-sulfur bacteria, are consistent with this being thermally adapted. Expand
Stopped‐flow studies of the reaction of d‐tartronate semialdehyde‐2‐phosphate with human neuronal enolase and yeast enolase 1
TLDR
The results are interpreted in terms of a catalytically significant interaction between the two subunits of these enzymes, which reduces the relative magnitude of the slower reaction. Expand
A study of the in-vitro regulation of phosphoenolpyruvate carboxylase from the epidermis of Commelina communis by malate and glucose-6-phosphate
TLDR
Phosphoenolpyruvate (PEP) carboxylase activity in epidermal extracts of Commelina communis has been compared in the presence of malate and glucose-6-phosphate and no increase in enzyme activity was observed, although there was a slight decrease in the Km. Expand
Thermodynamics and Kinetics of Glycolytic Reactions. Part I: Kinetic Modeling Based on Irreversible Thermodynamics and Validation by Calorimetry
TLDR
The use of irreversible thermodynamics is proposed to describe the kinetic approximation to the equilibrium state in a consistent way with very few adjustable parameters and the temperature dependence of the kinetic parameter fulfilled the Arrhenius relation and the derived activation energies are plausible. Expand
Featured Species-Specific Loops Are Found in the Crystal Structure of Mhp Eno, a Cell Surface Adhesin From Mycoplasma hyopneumoniae
TLDR
Results show that Mhp Eno has specific structural characteristics and acts as a multifunctional adhesin on the Mycoplasma hyopneumoniae cell surface and can recognize and interact with plasminogen with high affinity. Expand
Thermodynamics and Kinetics of Glycolytic Reactions. Part II: Influence of Cytosolic Conditions on Thermodynamic State Variables and Kinetic Parameters
TLDR
It is shown that the interplay of thermodynamic modeling and calorimetric process monitoring allows a fast and reliable quantification of the influence of cytosolic conditions on kinetic and thermodynamic parameters. Expand
Enolases: storage compounds in seeds? Evidence from a proteomic comparison of zygotic and somatic embryos of Cyclamen persicum Mill.
TLDR
The proteomes of somatic and zygotic embryos were characterised in a comparative approach and the glycolytic enzyme enolase was the protein most frequently detected and thus is supposed to play an important role in Cyclamen embryogenesis. Expand
The evolutionary origins and catalytic importance of conserved electrostatic networks within TIM‐barrel proteins
  • D. Livesay, David La
  • Biology, Medicine
  • Protein science : a publication of the Protein Society
  • 2005
TLDR
The evolutionary and catalytic importance of electrostatic networks in three ubiquitous metabolic enzymes: triosephosphate isomerase, enolase, and transaldolase are demonstrated using phylogenetic motifs (sequence fragments that parallel the overall familial phylogeny). Expand
An integrated proteomics reveals pathological mechanism of honeybee (Apis cerena) sacbrood disease.
TLDR
The data, for the first time, comprehensively decipher the molecular underpinnings of the viral infection of the Acc and are potentially helpful for sacbrood disease diagnosis and medicinal development for the prevention of this deadly viral disease. Expand

References

SHOWING 1-2 OF 2 REFERENCES
Studies on the enzyme enolase. I. Equilibrium studies.
by gest on O cber 20, 2017 hp://w w w .jb.org/ D ow nladed from Finn Wold and Clinton E. Ballou I. EQUILIBRIUM STUDIES STUDIES ON THE ENZYME ENOLASE: 1957, 227:301-312
  • J. Biol. Chem.,
  • 1957