SSCP primer design based on single-strand DNA structure predicted by a DNA folding program.

Abstract

To predict alterations in single-strand DNA mobility in non-denaturing electrophoretic gels, Zuker's RNA folding program was modified. Energy files utilized by the LRNA RNA folding algorithm were modified to emulate folding of single-strand DNA. Energy files were modified to disallow G-T base pairing. Stacking energies were corrected for DNA thermodynamics… (More)

Topics

6 Figures and Tables

Cite this paper

@article{Nielsen1995SSCPPD, title={SSCP primer design based on single-strand DNA structure predicted by a DNA folding program.}, author={D A Nielsen and A G Novoradovsky and David Goldman}, journal={Nucleic acids research}, year={1995}, volume={23 12}, pages={2287-91} }