SSCP analysis of long DNA fragments in low pH gel.

  title={SSCP analysis of long DNA fragments in low pH gel.},
  author={Yoji Kukita and Tomoko Tahira and S. S. Sommer and Kenshi Hayashi},
  journal={Human mutation},
  volume={10 5},
Sensitivity of single-strand conformation polymorphism analysis of PCR products (PCR-SSCP analysis) is known to be decreased when the DNA fragments are longer than 300 bp. We examined effects of buffer ions in an attempt to extend the length limit of the analysis. It has been noted that addition of glycerol to the gel containing Tris-borate buffer enhances the sensitivity, but the effects of glycerol have been left unexplained. We found that the effects of glycerol are caused by the reduction… CONTINUE READING

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