SPR evaluation of binding kinetics and affinity study of modified RNA aptamers towards small molecules.


Establishing an efficient method for evaluating the affinity changes after post-SELEX modification of aptamers is essential for broadening the application of these oligonucleotides in biosensing. This is especially challenging when the ligand is a small molecule. Changes in affinity upon partial or total replacement of 2'-OH with 2'-OMe groups in the ribose… (More)
DOI: 10.1016/j.talanta.2012.07.019


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