SPECT Imaging with 99mTc-Labeled EGFR-Specific Nanobody for In Vivo Monitoring of EGFR Expression

@article{Huang2008SPECTIW,
  title={SPECT Imaging with 99mTc-Labeled EGFR-Specific Nanobody for In Vivo Monitoring of EGFR Expression},
  author={Lieven Huang and Lea Olive Tchouate Gainkam and Vicky Caveliers and Chris Vanhove and Marleen Keyaerts and Patrick de Baetselier and Axel Bossuyt and Hilde Revets and Tony Lahoutte},
  journal={Molecular Imaging and Biology},
  year={2008},
  volume={10},
  pages={167-175}
}
Overexpression of the epidermal growth factor receptor (EGFR) occurs with high incidence in various carcinomas. The oncogenic expression of the receptor has been exploited for immunoglobulin-based diagnostics and therapeutics. We describe the use of a llama single-domain antibody fragment, termed Nanobody®, for the in vivo radioimmunodetection of EGFR overexpressing tumors using single photon emission computed tomography (SPECT) in mice. Fluorescence-activated cell sorting (FACS) analysis was… CONTINUE READING