Genomic and gene expression signature of the pre-invasive testicular carcinoma in situ
- BiologyCell and Tissue Research
The gene expression profile of CIS cells has remarkable similarity to that of embryonic stem cells and supports the long-standing hypothesis of an early developmental origin of CIS and testicular germ cell cancer.
Detection and characterization of chromosomal aberrations in human solid tumors using fluorescence in situ hybridization strategies
It is shown that chromosome paints specific for the common aberrant chromosomes, such as the Philadelphia chromosome, can be generated and made widely available and may find particular use in the analysis of complex or masked chromosomal translocations.
Generation of a panel of somatic cell hybrids containing fragments of human chromosome 12P by X-ray irradiation and cell fusion.
Cytogenetic analysis of 124 prospectively ascertained male germ cell tumors.
- MedicineCancer research
Cytological evidence of gene amplification in the form of homogeneously staining regions and/or double minutes was detected in 24% of extragonadal lesions, suggesting amplification of a gene(s) associated with metastatic progression of these tumors.
Primary mediastinal germ cell tumours: an immunohistochemical and molecular diagnostic approach
A diagnostic algorithm using immunohistochemical staining, with a focus on novel markers, and molecular analysis of isochromosome 12p [i( 12p], is developed.
Gains of 12p13.31 delay WNT-mediated initiation of hPSC differentiation and promote residual pluripotency in a cell cycle dependent manner
The results further refine the molecular mechanisms that underpin the exit from pluripotency and onset of differentiation and highlight the ability of genetically abnormal hPSC to escape correct differentiation and to form residual pluripotent cells, an important risk in the safe clinical translation of h PSC.
Standardization of Cell Culture Conditions and Routine Genomic Screening under a Quality Management System Leads to Reduced Genomic Instability in hPSCs
It is demonstrated that application of a QMS to standardize cell culture conditions and genomic monitoring routines leads to a striking improvement of genomic stability in hPSCs cultured in vitro, as evidenced by a reduced probability of potentially pathogenic chromosomal aberrations and subchromosomal genomic alterations.
Diverse Roles and Targets of miRNA in the Pathogenesis of Testicular Germ Cell Tumour
The gene regulation and function of miRNAs involved in TGCT pathogenesis is summarized to suggest they may act as either oncogene or tumour suppressors, and individual miRNA targets and downstream pathways in the context of TGCT development have been explored.
Clonal diversification and histogenesis of malignant germ cell tumours
It is found that the extensive diversification of tissues and genetic subclones in GCTs were not correlated and unifying features including the retention of fetal developmental transcripts across tissues, expression changes on chromosome 12p, and a conserved somatic evolutionary sequence of whole genome duplication followed by clonal diversification were preserved.
The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells
Current knowledge on how chromosomal aberrations influence the cell state is discussed and how this may impact the future of research and the cells’ clinical potential is discussed.