SPARC interacts with AMPK and regulates GLUT4 expression.

  title={SPARC interacts with AMPK and regulates GLUT4 expression.},
  author={Haiyan Song and Yuanyuan Guan and Liping Zhang and Kun Li and Chunli Dong},
  journal={Biochemical and biophysical research communications},
  volume={396 4},
AMP-activated protein kinase (AMPK) is a critical regulator of glucose metabolism. To elucidate the biochemical mechanisms by which AMPK regulates glucose and fat metabolism, we conducted a yeast two-hybrid screen to identify its interacting partners. A yeast two-hybrid system was used to screen a mouse embryo cDNA library for proteins able to bind mouse AMPK alpha 1. We also demonstrated an endogenous interaction between AMPK alpha 1 and its interacting partner by co-immunoprecipitation of the… CONTINUE READING