SNP genotyping using single-tube fluorescent bidirectional PCR.

  title={SNP genotyping using single-tube fluorescent bidirectional PCR.},
  author={Christy M Waterfall and Benjamin D Cobb},
  volume={33 1},
          80, 82-4, 86 passim
SNP genotyping is a well-populatedfield with a large number of assay formats offering accurate allelic discrimination. However, there remains a discord between the ultimate goal of rapid, inexpensive assays that do not require complex design considerations and involved optimization strategies. We describe the first integration of bidirectional allele-specific amplification, SYBR Green I, and rapid-cycle PCR to provide a homogeneous SNP-typing assay. Wild-type, mutant, and heterozygous alleles… CONTINUE READING