SIMAC (sequential elution from IMAC), a phosphoproteomics strategy for the rapid separation of monophosphorylated from multiply phosphorylated peptides.

@article{Thingholm2008SIMACE,
  title={SIMAC (sequential elution from IMAC), a phosphoproteomics strategy for the rapid separation of monophosphorylated from multiply phosphorylated peptides.},
  author={Tine E. Thingholm and Ole N Jensen and Phillip J Robinson and Martin R Larsen},
  journal={Molecular & cellular proteomics : MCP},
  year={2008},
  volume={7 4},
  pages={661-71}
}
  • Tine E. Thingholm, Ole N Jensen, +1 author Martin R Larsen
  • Published 2008 in Molecular & cellular proteomics : MCP
The complete analysis of phosphoproteomes has been hampered by the lack of methods for efficient purification, detection, and characterization of phosphorylated peptides from complex biological samples. Despite several strategies for affinity enrichment of phosphorylated peptides prior to mass spectrometric analysis, such as immobilized metal affinity chromatography or titanium dioxide, the coverage of the phosphoproteome of a given sample is limited. Here we report a simple and rapid strategy… CONTINUE READING
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