SERS-based immunoassay using a gold array-embedded gradient microfluidic chip.

@article{Lee2012SERSbasedIU,
  title={SERS-based immunoassay using a gold array-embedded gradient microfluidic chip.},
  author={Moonkwon Lee and Kangsun Lee and Ki Hyung Kim and Kwang W. Oh and Jaebum Choo},
  journal={Lab on a chip},
  year={2012},
  volume={12 19},
  pages={
          3720-7
        }
}
Here we report the development of a programmable and fully automatic gold array-embedded gradient microfluidic chip that integrates a gradient microfluidic device with gold-patterned microarray wells. This device provides a convenient and reproducible surface-enhanced Raman scattering (SERS)-based immunoassay platform for cancer biomarkers. We used hollow gold nanospheres (HGNs) as SERS agents because of their highly sensitive and reproducible characteristics. The utility of this platform was… 
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References

SHOWING 1-10 OF 44 REFERENCES
On-chip immunoassay using surface-enhanced Raman scattering of hollow gold nanospheres.
TLDR
A novel microfluidic sensor with functional internal structures that can replace the set of microwells or microtubes used in manual serial dilutions that have been traditionally used in enzyme-linked immunosorbent assay (ELISA)-type assays has been designed and fabricated.
Highly reproducible immunoassay of cancer markers on a gold-patterned microarray chip using surface-enhanced Raman scattering imaging.
A portable surface-enhanced Raman scattering sensor integrated with a lab-on-a-chip for field analysis.
TLDR
An integrated real-time sensing system that uses a portable Raman spectrometer and a micropillar array chip has been developed for field analysis and offers a rapid and reproducible trace detection capability for hazardous materials in the field.
A reproducible surface-enhanced raman spectroscopy approach. Online SERS measurements in a segmented microfluidic system.
TLDR
This report reports on the online application of surface-enhanced micro-Raman spectroscopy for the detection and quantization of analytes in a liquid/liquid segmented microfluidic system and reaches in a reproducible quantification of analyzetes with the SERS technique.
Immunoassay readout method using extrinsic Raman labels adsorbed on immunogold colloids.
TLDR
It is demonstrated in a dualanalyte sandwich assay, in which two different antibodies covalently bound to a solid substrate specifically capture two different antigens from an aqueous sample, that two different antigenic species can be detected simultaneously.
SERS biodetection using gold-silica nanoshells and nitrocellulose membranes.
We have developed a rapid, reproducible, easy to execute, surface enhanced Raman scattering (SERS) method for detection of low volumes and total amounts of biological antigens using an analyte…
Surface enhanced Raman spectroscopy for microfluidic pillar arrayed separation chips.
TLDR
This design is the first integrated chip based microfluidic device to combine pressure driven separation capability with real time SERS detection and demonstrates the ability to collect distinctive SERS spectra with or without complete resolution of chromatographic bands.
Highly sensitive immunoassay of lung cancer marker carcinoembryonic antigen using surface-enhanced Raman scattering of hollow gold nanospheres.
TLDR
A quick and reproducible surface-enhanced Raman scattering (SERS)-based immunoassay technique, using hollow gold nanospheres (HGNs) and magnetic beads, has been developed, which overcomes the problem of slow immunoreaction caused by the diffusion-limited kinetics on a solid substrate.
Label-free reading of microarray-based immunoassays with surface plasmon resonance imaging.
TLDR
This work presents a means of fabricating microarrays with controlled surface density of antigens at a gold surface for surface plasmon resonance (SPR) imaging experiments and provides both quantitative and qualitative evaluation of antibody binding in a label free format.
Low-level detection of viral pathogens by a surface-enhanced Raman scattering based immunoassay.
TLDR
A diagnostic test employing monoclonal antibodies for the selective extraction of viral pathogens from a sample in a chip-scale, sandwich immunoassay format has been developed using surface-enhanced Raman scattering (SERS) as a readout method, which has a linear dynamic range and limit of detection of 1.5 x 10(8) viruses/mL.
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