SERCA structural dynamics induced by ATP and calcium.

  title={SERCA structural dynamics induced by ATP and calcium.},
  author={Benjamin Mueller and Min Zhao and Igor V Negrashov and Roberta Bennett and David D Thomas},
  volume={43 40},
We have used time-resolved phosphorescence anisotropy (TPA) to probe rotational dynamics of the rabbit skeletal sarcoplasmic reticulum Ca-ATPase (SERCA), to test the hypothesis, generated from X-ray crystallography, that large-scale structural changes are induced by Ca in this system. Previous TPA studies on SERCA used primarily erythrosin 5'-isothiocyanate (ErITC), which binds to the nucleotide-binding domain and inactivates the enzyme. To investigate rotational dynamics of the active enzyme… CONTINUE READING

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