SEPARATION OF MITOCHONDRIAL MEMBRANES OF NEUROSPORA CRASSA II . Submitochondrial Localization of the Isoleucine-Valine Biosynthetic Pathway

@inproceedings{2003SEPARATIONOM,
  title={SEPARATION OF MITOCHONDRIAL MEMBRANES OF NEUROSPORA CRASSA II . Submitochondrial Localization of the Isoleucine-Valine Biosynthetic Pathway},
  author={},
  year={2003}
}
  • Published 2003
Separation of Neurospora mitochondrial outer membranes from the inner membrane/ matrix fraction was effected by digitonin treatment and discontinuous density gradient centrifugation . The solubilization of four isoleucine-valine biosynthetic enzymes was studied as a function of digitonin concentration and time of incubation in the detergent . The kinetics of the appearance of valine biosynthetic function in fractions outside of the inner membrane/ matrix fraction, coupled with enzyme… CONTINUE READING