SDS -PAGE and Western Blotting Techniques.


The goal of Western blotting, or more correctly, immunoblotting, is to identify with a specific antibody a particular antigen within a complex mixture of proteins that has been fractionated in a polyacrylamide gel and immobilized onto a membrane. Immunoblotting can be used to determine a number of important characteristics of protein antigens-the presence… (More)
DOI: 10.1385/1-59259-136-1:145


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