SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation

@article{Budnik2018SCoPEMSMS,
  title={SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation},
  author={Bogdan Budnik and Ezra Levy and Guillaume Harmange and Nikolai Slavov},
  journal={Genome Biology},
  year={2018},
  volume={19}
}
Some exciting biological questions require quantifying thousands of proteins in single cells. To achieve this goal, we develop Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS) and validate its ability to identify distinct human cancer cell types based on their proteomes. We use SCoPE-MS to quantify over a thousand proteins in differentiating mouse embryonic stem cells. The single-cell proteomes enable us to deconstruct cell populations and infer protein abundance relationships. Comparison… Expand
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