SCP1 encodes an actin-bundling protein in yeast.


The association of F-actin (filamentous actin) with a large number of binding proteins is essential for cellular function. Actin-binding proteins control the dynamics of actin filaments, nucleate new filaments and facilitate formation of higher-order structures such as actin bundles. The yeast gene SCP1 encodes a small protein with significant homology to mammalian SM22/transgelin. We have investigated the role of Scp1p in budding yeast to probe the fundamental role of this family of proteins. Here, we demonstrate that Scp1p binds to F-actin and induces the formation of tight F-actin bundles in vitro. Deletion of SCP1 in yeast lacking the actin-bundling protein, fimbrin (Sac6p), exacerbates the disrupted actin phenotype and enhances latrunculin-A sensitivity. Furthermore, Scp1p co-localizes with actin in cortical patches and its localization is lost in the presence of latrunculin-A. Our data support a role for Scp1p in bundling actin filaments and, in concert with Sac6p, acting as a second actin-bundling activity crucial to the stability of the yeast actin cytoskeleton.


Citations per Year

121 Citations

Semantic Scholar estimates that this publication has 121 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Winder2003SCP1EA, title={SCP1 encodes an actin-bundling protein in yeast.}, author={Steven J. Winder and Thomas J. Jess and Kathryn R Ayscough}, journal={The Biochemical journal}, year={2003}, volume={375 Pt 2}, pages={287-95} }