SCFGrr1-mediated ubiquitination of Gis4 modulates glucose response in yeast.

@article{Rue2005SCFGrr1mediatedUO,
  title={SCFGrr1-mediated ubiquitination of Gis4 modulates glucose response in yeast.},
  author={Janna La Rue and Sara A. Tokarz and Stefan Lanker},
  journal={Journal of molecular biology},
  year={2005},
  volume={349 4},
  pages={685-98}
}
The F box protein Grr1 is the substrate specificity-determinant of the SCF(Grr1) E3 ubiquitin ligase complex. Genetic analyses of Grr1 mutants have implicated Grr1 in glucose repression, specifically with regard to expression of the SUC2 transcript. To better understand Grr1, we screened for substrates using a mutant version of Grr1 that should not associate with the SCF complex. We identified Gis4 as a novel Grr1 substrate. Gis4 was originally isolated as a multi-copy suppressor of a Gal… CONTINUE READING