SASI-Seq: sample assurance Spike-Ins, and highly differentiating 384 barcoding for Illumina sequencing

@inproceedings{Quail2013SASISeqSA,
  title={SASI-Seq: sample assurance Spike-Ins, and highly differentiating 384 barcoding for Illumina sequencing},
  author={Michael A. Quail and Miriam Smith and David K. Jackson and Steven R. Leonard and Thomas Skelly and Harold P Swerdlow and Yong Xi Gu and Peter F. Ellis},
  booktitle={BMC Genomics},
  year={2013}
}
A minor but significant fraction of samples subjected to next-generation sequencing methods are either mixed-up or cross-contaminated. These events can lead to false or inconclusive results. We have therefore developed SASI-Seq; a process whereby a set of uniquely barcoded DNA fragments are added to samples destined for sequencing. From the final sequencing data, one can verify that all the reads derive from the original sample(s) and not from contaminants or other samples. By adding a mixture… CONTINUE READING

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Proceedings of the National Academy of Sciences of the United States of America • 2012
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