SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells

@article{Leidel2005SAS6DA,
  title={SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells},
  author={S. Leidel and M. Delattre and L. Cerutti and K. Baumer and P. G{\"o}nczy},
  journal={Nature Cell Biology},
  year={2005},
  volume={7},
  pages={115-125}
}
The mechanisms that ensure centrosome duplication are poorly understood. In Caenorhabditis elegans, ZYG-1, SAS-4, SAS-5 and SPD-2 are required for centriole formation. However, it is unclear whether these proteins have functional homologues in other organisms. Here, we identify SAS-6 as a component that is required for daughter centriole formation in C. elegans. SAS-6 is a coiled-coil protein that is recruited to centrioles at the onset of the centrosome duplication cycle. Our analysis… Expand
Phosphorylation of SAS-6 by ZYG-1 is critical for centriole formation in C. elegans embryos.
TLDR
It is demonstrated that the kinase ZYG-1 phosphorylates the coiled-coil protein SAS-6 at serine 123 in vitro and established that such phosphorylation ensures the maintenance of SAS-7 at the emerging centriole and thus for faithful cell division. Expand
PP2A phosphatase acts upon SAS-5 to ensure centriole formation in C. elegans embryos.
TLDR
It is demonstrated that Protein Phosphatase 2A (PP2A) is also critical for centriole formation in C. elegans embryos and proposed that PP2A-mediated loading of SAS-6 proteins is critical at the onset of centrioles formation. Expand
Sequential Protein Recruitment in C. elegans Centriole Formation
TLDR
The order in which these five proteins are recruited to centrioles is established, and the presence of SAS-5 and SAS-6 allows diminution of centriolar ZYG-1, and astral microtubules appear dispensable for theCentriolar recruitment of all five proteins. Expand
SAS-1 Is a C2 Domain Protein Critical for Centriole Integrity in C. elegans
TLDR
This work establishes that sas-1 encodes a C2 domain containing protein that localizes to centrioles in C. elegans, and which can bind and stabilize microtubules when expressed in human cells, and uncovers that SAS-1 is related to C2CD3, a protein required for complete centriole formation inhuman cells and affected in a type of oral-facial-digital (OFD) syndrome. Expand
The C. elegans F-box proteins LIN-23 and SEL-10 antagonize centrosome duplication by regulating ZYG-1 levels
TLDR
In C. elegans, similar to Drosophila and humans, it is found that the Slimb/&bgr;TrCP homolog LIN-23 regulates ZYG-1 levels, and it is shown that a second F-box protein, SEL-10, also contributes to ZYg-1 regulation, which suggests these proteins function cooperatively. Expand
Protein phosphatase 2A-SUR-6/B55 regulates centriole duplication in C. elegans by controlling the levels of centriole assembly factors.
TLDR
It is found that the PP2A catalytic subunit LET-92, the scaffolding subunit PAA-1, and the B55 regulatory subunit SUR-6 function together to positively regulate centriole assembly and promote centrioles assembly by protecting ZYG-1 and SAS-5 from degradation. Expand
Drosophila Spd-2 Recruits PCM to the Sperm Centriole, but Is Dispensable for Centriole Duplication
TLDR
DSpd-2 appears to have a particularly important role in recruiting PCM to the sperm centriole, and hence for microtubule nucleation and pronuclear fusion. Expand
The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation
TLDR
This work reveals that two distinct self-associating domains are necessary to form higher-order oligomers of SAS-5: a trimeric coiled coil and a novel globular dimeric Implico domain, indicating that large SAS- 5 assemblies are necessary for function in vivo. Expand
APC/CFZR-1 Controls SAS-5 Levels to Regulate Centrosome Duplication in Caenorhabditis elegans
TLDR
This work reports that FZR-1, the Caenorhabditis elegans homolog of Cdh1/Hct1/Fzr, a co-activator of the anaphase promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase, functions as a negative regulator of centrosome duplication in the Caesarean embryo. Expand
APC/CFZR-1 Controls SAS-5 Levels To Regulate Centrosome Duplication in Caenorhabditis elegans
TLDR
FZR-1, the Caenorhabditis elegans homolog of Cdh1/Hct1/Fzr, a coactivator of the anaphase promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase, functions as a negative regulator of centrosome duplication in the C. elegans embryo. Expand
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TLDR
Fluorescence recovery after photobleaching experiments with green fluorescent protein fused to SAS-5 (GFP–SAS-5) demonstrated that the protein shuttles between centrioles and the cytoplasm throughout the cell cycle, and partial RNA-interference-mediated inactivation experiments suggest that both sas-5 and zyg-1 are dose-dependent regulators of centrosome duplication. Expand
SAS-4 is essential for centrosome duplication in C elegans and is recruited to daughter centrioles once per cell cycle.
TLDR
FRAP experiments with GFP-SAS-4 transgenic embryos reveal that SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication. Expand
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TLDR
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The Caenorhabditis elegans Centrosomal Protein SPD-2 Is Required for both Pericentriolar Material Recruitment and Centriole Duplication
TLDR
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TLDR
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TLDR
The zyg-1 gene of Caenorhabditis elegans is an essential regulator of centrosome duplication, a protein kinase specifically required for daughter centriole formation that localizes transiently to centrosomes and acts at least one cell cycle prior to each spindle assembly event. Expand
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TLDR
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TLDR
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