SARS-CoV-2 proteases cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species and the search for reservoir hosts

@article{Moustaqil2020SARSCoV2PC,
  title={SARS-CoV-2 proteases cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species and the search for reservoir hosts},
  author={Mehdi Moustaqil and Emmanuelle Ollivier and Hsin-Ping Chiu and Sarah Van Tol and Paulina Rudolffi-Soto and Christian S. Stevens and Akshay Bhumkar and Dominic J.B. Hunter and Alexander N. Freiberg and David A Jacques and Benhur Lee and Emma Sierecki and Yann Gambin},
  journal={bioRxiv},
  year={2020}
}
The genome of SARS-CoV-2 (SARS2) encodes for two viral proteases (NSP3/ papain-like protease and NSP5/ 3C-like protease or major protease) that are responsible for cleaving viral polyproteins for successful replication. NSP3 and NSP5 of SARS-CoV (SARS1) are known interferon antagonists. Here, we examined whether the protease function of SARS2 NSP3 and NSP5 target proteins involved in the host innate immune response. We designed a fluorescent based cleavage assay to rapidly screen the protease… Expand
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