SAR11 marine bacteria require exogenous reduced sulphur for growth

  title={SAR11 marine bacteria require exogenous reduced sulphur for growth},
  author={H. James Tripp and Joshua B. Kitner and Michael S. Schwalbach and John William Dacey and Larry J. Wilhelm and Stephen J. Giovannoni},
Sulphur is a universally required cell nutrient found in two amino acids and other small organic molecules. All aerobic marine bacteria are known to use assimilatory sulphate reduction to supply sulphur for biosynthesis, although many can assimilate sulphur from organic compounds that contain reduced sulphur atoms. An analysis of three complete ‘Candidatus Pelagibacter ubique’ genomes, and public ocean metagenomic data sets, suggested that members of the ubiquitous and abundant SAR11… 
One Carbon Metabolism in SAR11 Pelagic Marine Bacteria
Genetically conserved genes in SAR11 subgroup Ia (Candidatus Pelagibacter ubique) genomes encode pathways for the oxidation of a variety of one-carbon compounds and methyl functional groups from methylated compounds, supporting the conclusion that C1 oxidation might be a significant conduit by which dissolved organic carbon is recycled to CO2 in the upper ocean.
Methane production by phosphate-starved SAR11 chemoheterotrophic marine bacteria.
Pelagibacterales bacteria are identified as a source of biogenic methane and phosphate starvation of chemoheterotrophic bacteria in the long-observed methane supersaturation in oxygenated waters.
Sulfur metabolites that facilitate oceanic phytoplankton–bacteria carbon flux
The discovery of an abundant and diverse suite of marine bacteria with the genetic capacity for DOS transformation argues for an important role for sulfur metabolites in the pelagic ocean carbon cycle.
Nutrient requirements for growth of the extreme oligotroph ‘Candidatus Pelagibacter ubique’ HTCC1062 on a defined medium
Chemoheterotrophic marine bacteria of the SAR11 clade are Earth’s most abundant organisms. Following the first cultivation of a SAR11 bacterium, ‘Candidatus Pelagibacter ubique’ strain HTCC1062 (Ca.
The presence of the glycolysis operon in SAR11 genomes is positively correlated with ocean productivity.
A multiphasic exploration of carbohydrate utilization among SAR11 isolates from the Northeast Pacific Ocean and the Sargasso Sea indicated that glycolysis is a variable metabolic property of SAR11 metabolism and suggest that gly colytic SAR11 are more common in productive marine environments.
Elemental content and stoichiometry of SAR11 chemoheterotrophic marine bacteria
We measured the carbon, nitrogen, and phosphorus content and production of cultured SAR11 cells in the genus Pelagibacter, from members of the 1a.1 and 1a.3 lineages, which are adapted to productive
The abundant marine bacterium Pelagibacter simultaneously catabolizes dimethylsulfoniopropionate to the gases dimethyl sulfide and methanethiol
It is reported that Pelagibacter HTCC1062 produces the gas methanethiol, and that a second DMSP catabolic pathway, mediated by a cupin-like DMSP lyase, DddK, simultaneously shunts as much as 59% of DMSP uptake to dimethyl sulfide production.
On the molecular diversity of dimethylsulphoniopropionate catabolism by marine bacteria
A novel DMSP lyase is identified (DddK), which catalyses the cleavage of DMSP into acrylate and dimethyl sulphide (DMS) in the DMS-producing Candidatus Pelgaibacter ubique HTCC1062 - one of the most prolific bacteria on this planet.
Cobalamin Scarcity Modifies Carbon Allocation and Impairs DMSP Production Through Methionine Metabolism in the Haptophyte Microalgae Tisochrysis lutea
The results highlight the interconnection of carbon and DMSP metabolisms through the cobalamin-dependent methionine synthesis by showing that cobalam scarcity impacts the mechanisms of carbon allocation and reduces DMSP quota.
Unusual Sulfur Requirements During Laboratory Growth of Luteibacter
It is shown that some Luteibacter strains cannot utilize sulfate as a sulfur source, likely due to loss of genes encoding transport proteins, which could provide a means to engineer better symbiotic relationships between bacteria and fungi that may be relevant for agriculture.


Contribution of SAR11 Bacteria to Dissolved Dimethylsulfoniopropionate and Amino Acid Uptake in the North Atlantic Ocean
The contribution of SAR11 bacteria to amino acid assimilation was greater than would be expected based on their overall abundance, implying that SAR11acteria outcompete other prokaryotes for these labile compounds.
Dimethylsulfoniopropionate and Methanethiol Are Important Precursors of Methionine and Protein-Sulfur in Marine Bacterioplankton
Findings indicate that the demethylation/demethiolation pathway of DMSP degradation is important for sulfur assimilation and that MeSH is a key intermediate in the pathway leading to protein sulfur.
Assimilatory Sulfur Metabolism in Marine Microorganisms: Sulfur Metabolism, Protein Synthesis, and Growth of Alteromonas luteo-violaceus and Pseudomonas halodurans During Perturbed Batch Growth
Sulfate incorporation into residue protein can be used as an accurate measurement of de novo protein synthesis in these bacteria and is used as a measurement of bacterial growth in natural environments.
Structural and Regulatory Genes Required to Make the Gas Dimethyl Sulfide in Bacteria
It is demonstrated that the bacterial gene dddD is required for this process and that its transcription is induced by the DMSP substrate, and the inferred enzymatic mechanism for DMS liberation involves an initial step in which DMSP is modified by addition of acyl coenzyme A, rather than the immediate release of DMS by a DMSP lyase, the previously suggested mechanism.
Genome sequence of the deep-sea γ-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy
  • S. HouJimmy H. Saw M. Alam
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2004
The report of the complete genome sequence of the deep-sea γ-proteobacterium, Idiomarina loihiensis, isolated recently from a hydrothermal vent at 1,300-m depth on the Lōihi submarine volcano, Hawaii, reveals an integrated mechanism of metabolic adaptation to the constantly changingdeep-sea hydroThermal ecosystem.
Cultivation of the ubiquitous SAR11 marine bacterioplankton clade
The isolation of representatives of the SAR11 clade is reported, which indicates that diverse uncultivated microbial taxa dominate most natural ecosystems, which has prompted widespread efforts to elucidate the geochemical activities of these organisms without the benefit of cultures for study.
Genome Streamlining in a Cosmopolitan Oceanic Bacterium
P. ubique, the first cultured member of the SAR11 clade, has the smallest genome and encodes the smallest number of predicted open reading frames known for a free-living microorganism.
Two halophilic Ectothiorhodospira strains with unusual morphological, physiological and biochemical characters
Two strains belonging to the genus Ectothiorhodospira were isolated from enrichment cultures inoculated with sulfide-containing samples from the saltern of Trapani: neither of the two strains is capable of assimilatory sulfate reduction and neither grows in the dark.
Functional genomics and expression analysis of the Corynebacterium glutamicum fpr2-cysIXHDNYZ gene cluster involved in assimilatory sulphate reduction
The high degree of conservation of this cluster among the Actinomycetales supports the hypothesis that a different metabolic pathway for the reduction of inorganic sulphur compounds than that known from the well-studied model organisms E. coli and B. subtilis is used by members of this order.
Die Verwertung von molekularem Wasserstoff durch Chlorobium thiosulfatophilum
Summary1.All hydrogenase-positive Chlorobium-strains (12 out of 17 strains tested) can grow to some extent with cysteine as a sulfur source, when molecular hydrogen is electron donor. An assimilatory