SAK/PLK4 Is Required for Centriole Duplication and Flagella Development

@article{BettencourtDias2005SAKPLK4IR,
  title={SAK/PLK4 Is Required for Centriole Duplication and Flagella Development},
  author={M{\'o}nica Bettencourt-Dias and Ana Rodrigues-Martins and Lee Carpenter and Maria Giovanna Riparbelli and L. Lehmann and Melanie K. Gatt and Nunes Maria Do Carmo and François Balloux and Giuliano Callaini and David M. Glover},
  journal={Current Biology},
  year={2005},
  volume={15},
  pages={2199-2207}
}

Figures from this paper

Centrosome biogenesis and number: mechanisms of control: determination of SAK/PLK4 interactors

TLDR
An RNAi screen for novel SAK substrates and interactors whose depletion could affect centrosome duplication suggests the existence of one or several novel protein interactions and pathways through which SAK is playing its role.

Flies without Centrioles

Asterless is a scaffold for the onset of centriole assembly

TLDR
Independent functions for Asl as a scaffold for Plk4 and Sas-4 that facilitates self-assembly and duplication of the centriole and organization of pericentriolar material are identified.

Klp10A modulates the localization of centriole-associated proteins during Drosophila male gametogenesis

TLDR
It is demonstrated that the loss of Klp10A modifies the distribution of typical proteins involved in centriole assembly and function and the cartwheel is lacking or it appears abnormal in mutant centrioles, suggesting that this structure may spatially delimit protein localization.

Establishment and mitotic characterization of new Drosophila acentriolar cell lines from DSas-4 mutant

TLDR
Six new Drosophila cell lines derived from Drosophile homozygous mutants for DSas-4, a protein essential for centriole biogenesis are developed, with the potential use of these cell lines for the molecular dissection of anastral pathways in spindle and centrosome assembly.

Control of mitotic and meiotic centriole duplication by the Plk4-related kinase ZYG-1

TLDR
Differences in the regulation of mitotic and meiotic centrosome duplication, particularly with regard to ZYG-1 activity, are revealed, and an important role for centrosomes in spermatid formation is revealed.

Centriole duplication

TLDR
This work discusses how this autoregulatory feedback loop acts to limit the damaging effects caused by too much or too little Plk4, a low abundance protein whose stability is linked to the activity of the enzyme.

Cell-cycle-regulated expression of STIL controls centriole number in human cells

TLDR
It is shown that STIL is recruited to nascent daughter centrioles at the onset of centriole duplication and degraded, in an APC/CCdc20–Cdh1-dependent manner, upon passage through mitosis.
...

References

SHOWING 1-10 OF 60 REFERENCES

Sak/Plk4 and mitotic fidelity

TLDR
It is suggested that Sak provides feedback to cell cycle regulators, and thereby precision to the switch-like transitions of centrosome duplication and exit-from-mitosis to contribute to improvements in cancer risk assessment and novel therapies.

The Polo kinase Plk4 functions in centriole duplication

TLDR
It is identified that Plk4 is required — in cooperation with Cdk2, CP110 and Hs-SAS6 — for the precise reproduction of centrosomes during the cell cycle and this findings provide an attractive explanation for the crucial function of PlK4 in cell proliferation.

Late mitotic failure in mice lacking Sak, a polo-like kinase

Metaphase Arrest with Centromere Separation in polo Mutants of Drosophila

TLDR
Two strongly hypomorphic mutations of polo are reported that arrest cells of the larval brain at a point in metaphase when the majority of sister kinetochores have separated by between 20–50% of the total spindle length in intact cells.

The Drosophila pericentrin-like protein is essential for cilia/flagella function, but appears to be dispensable for mitosis

TLDR
It is shown that the sensory cilia of these neurons are malformed and the neurons are nonfunctional in d-plp mutants, a phenotype usually associated with defects in mechanosensory neuron function, and that D-PLP is essential for the formation of functional cilia and flagella in flies.

Polo kinase and progression through M phase in Drosophila: a perspective from the spindle poles

TLDR
Genes for the mitotic kinases Polo and Aurora A were first identified in Drosophila through screens of maternal effect lethal mutations for defects in spindle pole behaviour and point to functions at the metaphase to anaphase transition and in cytokinesis.

Zygotic development without functional mitotic centrosomes

polo encodes a protein kinase homolog required for mitosis in Drosophila.

TLDR
It is shown that mutation in polo leads to a variety of abnormal mitoses in Drosophila larval neuroblasts, and the sequence shows that polo encodes a 577-amino-acid protein with an amino-terminal domain homologous to a serine-threonine protein kinase.

Plk4 haploinsufficiency causes mitotic infidelity and carcinogenesis

TLDR
Using the in vivo model of partial hepatectomy to induce synchronous cell cycle entry, it was determined that the precise regulation of cyclins D1, E and B1 and of Cdk1 was impaired in Plk4+/− regenerating liver, and p53 activation and p21 and BubR1 expression were suppressed.
...