S100A8 and S100A9 are messengers in the crosstalk between epidermis and dermis modulating a psoriatic milieu in human skin.

Abstract

S100A8 and S100A9 are members of the S100A8 protein family that exist as homodimers and heterodimers in neutrophils, monocytes, and macrophages. Recent studies have shown the pivotal roles of S100A8 and S100A9 in the propagation of inflammation and keratinocyte proliferation in psoriasis. We found significant up-regulation of S100A8 and S100A9 secretion from keratinocytes in psoriatic lesions. To mimic the in vivo secretory conditions of S100A8 and S100A9 from psoriatic epidermal keratinocytes, we used the culture medium (CM) of S100A8 and S100A8/A9 adenovirus-transduced keratinocytes to investigate the functions of S100A8 and S100A9. We detected increased levels of various pro-inflammatory cytokines in the CM, including IL-8 and TNF-α, which are involved in aggravating psoriatic skin lesions, and IL-6 and members of the CXCL family of pro-angiogenic cytokines. The CM increased immune cell migration and increased angiogenesis in human umbilical vein endothelial cells. In conclusion, we found that the upregulated production of S100A8 and S100A9 by psoriatic epidermal keratinocytes activated adjacent keratinocytes to produce several cytokines. Moreover, S100A8 and S100A9 themselves function as pro-angiogenic and chemotactic factors, generating a psoriatic milieu in skin.

DOI: 10.1016/j.bbrc.2012.05.162

4 Figures and Tables

05010015020132014201520162017
Citations per Year

176 Citations

Semantic Scholar estimates that this publication has 176 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Lee2012S100A8AS, title={S100A8 and S100A9 are messengers in the crosstalk between epidermis and dermis modulating a psoriatic milieu in human skin.}, author={Young Lee and Sunhyae Jang and Jeong-Ki Min and Kyungmin Lee and Kyung-Cheol Sohn and J Lim and Myung Im and Hae-Eul Lee and Young-Joon Seo and Chang Deok Kim and Jeung-Hoon Lee}, journal={Biochemical and biophysical research communications}, year={2012}, volume={423 4}, pages={647-53} }