S1 mapping of purified nascent transcripts of simian virus 40.

@article{Lycan1984S1MO,
  title={S1 mapping of purified nascent transcripts of simian virus 40.},
  author={D. Lycan and K. Danna},
  journal={Molecular and cellular biology},
  year={1984},
  volume={4 4},
  pages={
          625-33
        }
}
  • D. Lycan, K. Danna
  • Published 1984
  • Biology, Medicine
  • Molecular and cellular biology
  • We purified nascent simian virus 40 late transcripts by incubating viral transcriptional complexes, isolated from infected BSC-1 cells, in a reaction mixture that contained mercurated CTP; RNA molecules that had incorporated mercurated residues in vitro were isolated by sulfhydrylcellulose affinity chromatography. The nascent RNA was hybridized to an end-labeled HindIII C probe fragment (0.646 to 0.86 map unit), and the hybrids were analyzed by S1 mapping. Most of the products of digestion… CONTINUE READING