S1 and KH domains of polynucleotide phosphorylase determine the efficiency of RNA binding and autoregulation.

@article{Wong2013S1AK,
  title={S1 and KH domains of polynucleotide phosphorylase determine the efficiency of RNA binding and autoregulation.},
  author={Alexander G Wong and Kristina L. McBurney and Katharine J. Thompson and Leigh M. Stickney and George A. Mackie},
  journal={Journal of bacteriology},
  year={2013},
  volume={195 9},
  pages={2021-31}
}
To better understand the roles of the KH and S1 domains in RNA binding and polynucleotide phosphorylase (PNPase) autoregulation, we have identified and investigated key residues in these domains. A convenient pnp::lacZ fusion reporter strain was used to assess autoregulation by mutant PNPase proteins lacking the KH and/or S1 domains or containing point mutations in those domains. Mutant enzymes were purified and studied by using in vitro band shift and phosphorolysis assays to gauge binding and… CONTINUE READING