S-phase cell detection with a monoclonal antibody.

  title={S-phase cell detection with a monoclonal antibody.},
  author={Herbert Georg Miltenburger and G. W. Sachse and M G Schliermann},
  journal={Developments in biological standardization},
The use of a newly developed monoclonal antibody against Bromodeoxyuridine is demonstrated in a study with V79 Chinese hamster cells. It can replace radioactive material in cell cycle analysis studies in the way that BrdU incorporated into the DNA during S-phase is detected by immunofluorescence. By this technique the proportion of actively synthesizing S-phase cells can be determined. The proportion of such cells is a good measure with regard to the quality of a culture and/or medium in… CONTINUE READING

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